rabbit polyclonal ddr2 antibodies  (Santa Cruz Biotechnology)

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Representative image of DDR2 and collagen type I immunostaining in human breast cancer. ( A – C ): immunostaining of DDR2 in breast cancer cells ( A ), normal breast epithelium ( B ), and human heart as a positive control of DDR2 ( C ). ( D – F ): immunostaining of collagen type I in cancerous stroma ( D ), normal breast stroma ( E ), and human skin as a positive control of collagen type I ( F ). Bar = 100 µm, respectively.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Immunostaining, Positive Control

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinicopathological factors in 224 breast carcinomas.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinicopathological factors according to collagen type I status in 224 breast carcinomas.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinical outcomes of breast cancer patients (n = 224). ( A – D ): disease-free survival ( A , C ) and breast cancer-specific survival ( B , D ) according to DDR2 status ( A , B ) or DDR2/collagen type I combination status ( C , D ). ( E – H ): disease-free survival according to DDR2 status ( E , F ) or DDR2/collagen type I combination status ( G , H ) in the patients who received chemotherapy ( E , G ) or not ( F , H ).

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Uni- and multivariate analysis of disease-free survival.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 in the proliferation of human breast cancer cell lines in the presence of collagen type I. ( A ) Immunoblotting of exogenous DDR2 protein in MCF-7, MDA-MB-231, and T47D cells. ( B – D ): cell proliferation of MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( D ) transfected with an empty vector or DDR2-expressing vector in the absence or presence of collagen coating. ( E ) Immunoblotting of DDR2 in the cells transfected with siRNA against DDR2 (siDDR2-1, 2). ( F – H ): cell proliferation of MCF-7 ( F ), MDA-MB-231 ( G ), and T47D ( H ) transfected with siRNAs in the presence of collagen coating. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the empty vector, respectively.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 on the resistance to epirubicin in the breast cancer cell lines. ( A – C ): viability of MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( C ) transfected with an empty vector or DDR2-expressing vector under epirubicin treatment (500 nM for MCF-7; 250 nM for MDA-MB-231 and T47D). These cells were plated onto collagen-coated culture plates. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the empty vector, respectively. ( D – F ): viability of MCF-7 ( D ), MDA-MB-231 ( E ), and T47D ( F ) transfected with siRNA targeting DDR2 under epirubicin treatment. ( G , H ) mRNA and protein expression in chemosensitive parental cells and epirubicin-resistant cells ( G ; MCF-7 series, H ; MDA-MB-231 series). ** p < 0.01, respectively. ( I , J ) The effect of DDR2 inhibitor WRG-28 treatment (48 h) on the proliferation of chemosensitive parental cells and epirubicin-resistant cells ( F ; MCF-7 series, G ; MDA-MB-231 series).

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Transfection, Plasmid Preparation, Expressing

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 on the apoptosis of breast MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( C ). *** p < 0.001.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2

doi: 10.3892/etm.2019.7554

Figure Lengend Snippet: DDR2 positive expression levels before and after treatment in the three groups of rats (%).

Article Snippet: Type II collagenase was purchased from Sigma; Merck KGaA (Darmstadt, Germany); rabbit anti-mouse DDR2 polyclonal antibody was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA); TRIzol reagent was purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA); PCR fluorescence quantitative kit and reverse transcription kit were purchased from Dalian Takara Biotechnology Co., Ltd. (Dalian, China).

Techniques: Expressing, Transplantation Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2

doi: 10.3892/etm.2019.7554

Figure Lengend Snippet: Correlation analysis between MMP-13 mRNA and DDR2 in KOA rats. There was a positive correlation between MMP-13 mRNA and DDR2 in KOA rats (r=0.830, P<0.001). MMP-13, matrix metalloproteinase 13; DDR2, discoid domain receptor 2; KOA, knee osteoarthritis.

Article Snippet: Type II collagenase was purchased from Sigma; Merck KGaA (Darmstadt, Germany); rabbit anti-mouse DDR2 polyclonal antibody was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA); TRIzol reagent was purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA); PCR fluorescence quantitative kit and reverse transcription kit were purchased from Dalian Takara Biotechnology Co., Ltd. (Dalian, China).

Techniques: